Serum lipoprotein profile and concentration of dehydroepiandrosterone sulfate (DHEAS) in glucose-6-phosphate dehydrogenase-deficient subjects.

CLINICAL CHEMISTRY, Vol. 36, No. 2, 1990 393 automated ELISA plate reader and related the antigen concentration of the unknown plasma samples to the standard curve. Standard curves were constructed in triplicate on each plate to compensate for any plate-to-plate variation. The unknown plasma samples were run in triplicate at two dilutions, fiveand 20-fold. The lower limit of assay detection for EDP was 10 ngIL. CV for 10 determinations of a plasma sample at an EDP concentration of 0.4 .tgfL was 5.2% interbatch, 4.3% intrabatch; for 10 determinations at 20 ngfL, 1.4% interbatch, 1.2% intrabatch.